Composite

Part:BBa_K4501017:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)


Lamp2b-CD19-L


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1309
    Illegal XhoI site found at 2253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5655
    Illegal AgeI site found at 2050
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1015
    Illegal BsaI.rc site found at 2287
    Illegal SapI.rc site found at 1451


Design Notes

We performed a thorough computational modeling to unravel which of the different possibilities of the fusion protein was optimal in terms of CD19 specificity and construction viability. The part was split into 4 parts to be assembled by Golden Gate.


Source

Designed by ourselves.

References